Column Selection Chart

What's  on this page?

This HPLC column selection chart will help you choose the right HPLC column for your analysis or application. Simply determine the molecular weight of your analyte, and follow the chart downwards. The best suited HPLC separation mode and packing material is at the bottom of these charts.
 

Column selection chart: molecular weight < 2000 g/mol

water soluble organic solvent soluble
non-ionic ionic THF soluble hexane soluble methanol and methanol/water soluble
LC mode  (packing)  RP bonded (phenyl, CN, C8, C18) RP ionization control (phenyl, CN, C8, C18) RP polymeric RP paired-ion ion exchange (SAX, SCX) ion chromato-
graphy
small molecule size exclusion NP adsorbtion (Si) NP bonded (CN, NH2) RP bonded( CN, NH2, phenyl C8, C18)

Column selection chart: molecular weight > 2000 g/mol

water soluble organic solvent soluble
LC mode  (packing)  RP bonded (C18, phenyl, C4) aqeous gel filtration (SEC) ion exchange hydrophobic interaction affinity size exclusion chromatography
last update: augustus 11, 2007