Buffers: Preparation and details

What's on this page?

For buffer use in HPLC, I have collected data on the pKa, maximum buffer working range and UV cutoff. For an extended list of buffer ranges but rounded at 0.5 pH units, see the pKa/pKb tabel elsewhere on this site.
 

Buffer details for HPLC

 
buffer pKa (25°C) max.buffer range UV cutoff (nm)
TFA 0,3   210 (0.1%)
phosphate, pKa1 2,1 1.1-3.1 < 200
phosphate, pKa2 7,2 6.2-8.2 < 200
phosphate, pKa3 12,3 11.3-13.3 < 200
citrate, pKa1 3,1 2.1-4.1 230
citrate, pKa2 4,7 3.7-5.7 230
citrate, pKa3 6,4 5.4-7.4 230
carbonate, pKa1 6,1 5.1-7.1 < 200
carbonate, pKa2 10,3 9.3-11.3 < 200
formate 3,8 2.8-4.8 210 (10 mM)
acetate 4,8 3.8-5.8 210 (10 mM)
ammonia 9,3 8.3-10.3 200 (10 mM)
borate 9,2 8.2-10.2 n/a
TEA 10,8 9.8-11.8 < 200

More links...

  • A very good site about buffer preparation where you can even enter the ionic concentration, is here.
  • Alternative buffer preparation from ZirChrom.
  • Enter a pH and get back the right buffer at Reall.
 

Updates & Comments

Please use this form to notify me if you have any more data on the preparation of buffers.

http://www.raell.demon.co.uk/chem/calcs/LC_calc/index.htm

 

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last update: augustus 11, 2007